laser scanning confocal microscopy

Structural & Morphology Characterization (Light and acoustic microscopy)

The facility provides access to state-of-the-art imaging infrastructure incorporating the latest technology in multiphoton microscopy and optical imaging, with a mission of enabling researchers to visualize and monitor fundamental processes deep inside molecular structures including soft materials, living cells and organisms. Sophisticated laser scanning microscopic instrumentation, ultra-sensitive digital cameras and specialized fluorescence probes make it possible to visualize cellular events in real time down to the molecular level. In addition, two-photon microscopy allows unparalleled detail in soft matter imaging and is extremely useful in monitoring cellular processes in vivo, enabling prolonged observations that are not possible with classic confocal microscopes.

The facility comprises of two laser scanning confocal microscope units and a spinning disk confocal microscopy system and a NLO/DUO/InTune multiphoton microscope offering capabilities, such as FRAP, FRET, FLIM, DICM, FC/DF, FLIP and live cell Imaging. In addition a Hybrid Photoacoustic and Laser Scanning Confocal Microscopy workstation is in operation offering real time structural and functional imaging of optically opaque samples including soft materials, live cells, tumor spheroids and biomimetic tissue samples.

          provided at NFFA-Europe laboratories by:
Zeiss LSM 880 Airyscan
Zeiss LSM980
5x / 0.16 APO calibration LSM / Air . 10x / 0.3 Plan-Apochromat. 25x / 0.8 LD LCI Plan-Apochromat Imm Korr DIC M27 Oil. 40x / 1.20 C-Apochromat W Korr FCS Water
Diode UV 405 nm. Diode 488 nm. DPSS 561 nm. Diode 639 nm
PMT1, HyD, PMT3, PMT4 and PMT5 detectors. T-PMT transmitted light detector.
Confocal mode: Maximum lateral resolution (xy) : 200 nm. Maximum axial (xyz) resolution: 500 nm. Airyscan mode: Maximum lateral resolution (xy): 120 nm. Maximum axial (xyz) resolution: 350 nm
Piezoelectric and motorized stage
Incubation system: Thermostatic plate insert. Integral microscope incubator with temperature, CO2 and humidity control
Leica TCS SP5
10x/0.40 DRY UV. HCX PL APO CS 20x/0.70 DRY UV. HCX PL APO CS 40x/1.25 OIL UV. HCX PL APO lambda blue 63x/1.4 OIL UV. HCX PL APO CS 20x/0.70 IMM UV. HCX PL APO 63x/1.20 WATER
Diode UV 405 nm. Argon 458, 476, 488, 496 and 514 nm. DPSS 561 nm. HeNe 594 nm. HeNe 633 nm. Ti:Sapphire Mai TaiĀ® broadband (710-990 nm)
PMT1, HyD, PMT3, PMT4 and PMT5 detectors. PMT-Trans transmitted light detector. NDD1, NDD2 detectors
Maximum lateral resolution (xy) : 200 nm. Maximum axial (xyz) resolution: 500 nm
Galvanometric and motorized stage
Incubation system: Comprehensive microscope incubator, temperature controller, CO2 controller