Fourier Transform Infra Red Spectroscopy and Spectromicroscopy

Electronic & Chemical & Magnetic Characterization (Optical spectroscopy)

Though based on the same physical phenomena regarding the interaction between radiation and matter, today’s IR spectrometers have evolved in the way they irradiate the sample, replacing the former monochromators for interferometers, giving rise to the faster FT-IR spectroscopy.

Besides its classical application in chemical characterization, the use of infrared radiation has evolved giving rise to new techniques that go further:

VCD (Vibrational Circular Dichroism) is a technique that gives 3D information of molecules. It can be applied for determining the secondary structure of proteins and peptides, the purity of enantiomers and also their absolute configuration by comparison with previously reported data or with data obtained through theoretical simulations.

PM-IRRAS (Polarization Modulation-IR Reflection-Adsorption Spectroscopy) is a very useful technique for the analysis of ultrathin layers and coatings, monolayers and submonolayers and biomolecules, deposited on surfaces, especially for conductors (Au, Cu, Pd, alloys, etc). It allows for the study of not only the composition but also the organization, conformation and orientation of molecules on a given substrate. In addition, it is also useful for analysing phenomena affecting such surfaces, as could be corrosion processes. Thanks to the characteristics of this technique, samples can be measured without reference, giving rise to spectra free from atmospheric interferences such as carbon dioxide and water vapour

FT-IR Microscopy which allows for visible inspection of samples and to obtain FT-IR spectra by coupling all-reflective Vis-IR microscopes to a FT-IR spectrometer. It is useful for performing chemical characterisation in concrete points, and also for obtaining chemical maps of larger areas, with minimum spot sizes of 25-30 μm using conventional IR sources. Diffraction-limited resolution can be achieved exploiting the brightness advantage of IR Synchrotron Radiation (SR), for unveiling vibrational details at few microns in the Mid-IR regime. The non-damaging nature of IR SR and the use of IR-transparent fluidic devices permit the analysis of hydrated species, to follow sample dynamics and to work under physiological conditions at single cell level.

FT-IR Imaging  is a very versatile toll for chemical imaging of large sample areas, that takes advantage from bi-dimensional Focal Plane Arrays (FPA) detectors. The technique is ideally suited for the rapid analysis of a large variety of chemically heterogeneous samples, from slice tissues to polymer blends, in transmission, reflection and ATR mode.

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          provided at NFFA-Europe laboratories by:
CNR-DSCTM
Italy
CEA/LETI
France
C2N-CNRS
France
CSIC-CNM
Spain
CSIC-ICMAB
Spain
CSIC-ICMM
Spain
FORTH
Greece
ICN2
Spain
JRC - ISPRA
Italy
TUG
Italy
UMIL
Italy
EURONANOLAB
France
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          provided at Large Scale Facilities by:
ELETTRA
Italy
ALBA
Spain
ELETTRA
Italy
VERTEX 70v interferometer and Hyperion 3000 Vis/IR microscope @ Chemical and Life-Sciences branches of SISSI beamline at Elettra
Microscopy and Imaging in the MIR regime and with the newest VERTEX FM technology, for spectroscopy covering the FIR/THz and MIR in a single scan.
a. Halogen for the Near Infrared (NIR) b. Globar for the Mid Infrared (MIR)
a. KBr beamsplitter for MIR regime up to ~400 cm-1 b. CaF2 beamsplitter for NIR c. Ultra-wide range MIR-FIR beamsplitter (6000-50 cm-1)
a: DTGS, MCT and wide range DLaTGS detectors for spectroscopy measurements in the MIR and MIR-FIR regimes b: MCT-A, MCT-B and FPA detectors for microscopy and imaging in the MIR regime
a: Max spectral resolution: 0.5 cm-1 b: Max spatial resolution: diffraction limited (typically in the range of few microns in the MIR spectral range)
The branchline is well suited for biological and biomedical experiments, encompassing tissue imaging and single-cell analysis on both dehydrated and hydrated samples. Bulk materials and surface analysis can also be performed selecting the appropriate sampling techniques. a: For spectroscopy Transmission measurements on pellets and within Diamond Compression cells (available with 0.8 and 2 mm culet) Demountable liquid-cell T-controlled for transmission measurements of liquids Attenuated Total Reflectance (ATR) measurements i. Single Reflection ATR with Germanium, diamond and silicon IREs ii. Grazing angle single reflection ATR with Ge IRE iii. Multiple ATR with 25 reflection trapezoid IREs (Ge, Si, ZnSe, ...) 4. T-controlled Single Reflection monolithic ATR accessory for MIR-FIR measurements b: For Microscopy Transmission measurements on thin samples (slices) (15X and 36X objectives) Reflection/Transflection measurements Grazing Angle Reflection measurements Micro-ATR measurements
Room temperature and pressure microscopy measurements, with possibility to T-control from 10 to 90 °C. Spectroscopy measurements are possible also in vacuum (10-3 Torr) and in the same T-range.
a: Ready-to-use bio-compatible liquid cells for live cell analysis b: Possibility to design ad-hoc chips for reflection and transmission measurements in collaboration with potential users
CSIC-ICMM
Spain
Stardust: INFRA-ICE module: Transmission
IR spectroscopy (mid- and near IR) in transmission under ultra-high vacuum conditions and at low temperatures (T=15-300K)
Mid-IR: standard globar and a water-cooled high-power source. Near-IR: tungsten halogen lamp.
12800 cm^-1 - 850 cm^-1
For mid-IR: DLaTGS and MCT detectors. For near-IR: InGaAs detector.
Maximum dimensions: 15 x 15 mm^2. For low temperature experiments: 10 x 10 x 1 mm^2. Substrate needs to be IR transparent (e.g., KBr, ZnSe, CsI...).
Base pressure: 4 x 10^-10 mbar. Temperature control: 15 - 300 K. Gases up to 10^-6 mbar can be introduced in the sample chamber.
Quadrupole mass spectrometry (1 - 200 amu) for Thermal Programmed Desorption experiments (T = 15 - 300 K).
12800 cm^-1 - 850 cm^-1
Maximum resolution: 0.16 cm^−1
UV irradiation (Ly-alpha kine: 121.6 nm). Electron irradiation (1-500 meV). Ion irradiation (0.2 - 6 keV).
No
No
JRC - ISPRA
Italy
Micro-FTIR (Fourier Transform InfraRed Spectrometer coupled with a microscope)
FTIR stands for Fourier Transform InfraRed, a method of infrared spectroscopy. When IR radiation is passed through a material, some radiation is absorbed by the sample and some passes through (is transmitted). The resulting signal at the detector is a spectrum representing an absorption by molecular vibrations within the sample. The usefulness of infrared spectroscopy arises because different chemical structures (molecules, side groups, functionalities) produce different spectral fingerprints.