CzechNanoLab provides open access to the equipment and services in nanotechnology and materials science. CzechNanoLab consists of two sites, CEITEC Nano located in Brno, and the Laboratory of Nanostructures and Nanomaterials (LNSM), located in Prague. These two workplaces provide fast and easy access to cutting-edge equipment expertise for fabrication and analysis of nanostructures and nanomaterials. Researchers from both academic and industrial institutions from the Czech Republic and from abroad may access this infrastructure. Basic technologies and devices are available on both CzechNanoLab sites, in advanced and more demanding equipment the sites complement each other. CzechNanoLab also provides its services and expertise to other research infrastructures in the Czech Republic and is involved in European and global networks such as EuroNanoLab, IUVSTA (International Union for Vacuum Science, Technique and Applications) or AVS (American Vacuum Society).