High-Throuput Screening

Nano to Micro/Macro (in vitro assays and cell analysis)

High-throughput screening (HTS) on cell-based assays measures cellular responses via methods such as absorbance, fluorescence, and luminescence. Absorbance-based assays detect variations in the light absorption by cells, which can be indicative of cellular activity or proliferation. In fluorescence-based assays, fluorescent dyes are used to monitor specific cellular mechanisms or molecular interactions. Luminescence-based assays evaluate biological processes, often from enzyme-substrate reactions, by light emission detection.  Compared to biochemical or isolated target-based assays, HTS cell-based assays are able to evaluate compound effects on cellular models, which are more closely resemble physiological conditions. The evaluation of cellular response to compounds in question, leads to further research routes in understanding potential therapeutic effects, toxicity and mechanisms. Additionally, HTS cell-based assays could give information regarding cell signaling pathways, proliferation, apoptosis, differentiation.

High-throughput cell-based assays, has been further facilitated by automated liquid handling systems and robotic platforms, allowing the rapid screening of large libraries of compounds in short time period. The above advantages benefit the screening procedure, not only regarding time period, but also by contributing to the identification of potential drug candidates, novel targets and alternative pathways for drug development.

Cell-based assays used in high-throughput screening offer a powerful tool for evaluating compound effects on cellular mechanisms. They are crucial in fields of drug discovery and pathophysiology. With continuous improvements in technology and methodology, cell-based assays play a crucial role in accelerating biomedical research and development.

          provided at NFFA-Europe laboratories by:
HTS @ IBA/NCSR Demokritos
- Multi-parametric spectrophotometer suitable for rapid screening techniques by measuring absorption, fluorescence and chemiluminescence. - Multiplex analysis based on microbead flow cytometry analyzes hydrodynamic, optical and digital data allowing multiple simultaneous analysis of targets per sample or well. - Automated microplate handling system undertakes the repetitive fluid handling required in a high-performance experimental protocol with large number of wells or with multiple compounds. - Flow cytometry delivers reliable data for a variety of applications in cell samples. It provides precise and accurate data, making it an important tool in research and therapeutic contexts. - Cell culture Unit offers multiple cell culture facilities for: Mammalian & Insect cell cultures, Fungal cultures, Plant growth, Biobank of cell lines, Co-cultures and 3D cell systems, studies of cytotoxicity, cell death, cell proliferation, estrogenic activity, oxidative stress
Cell culture systems based on mammalian cells
Natural products’ extract, isolated compounds from chemical library.
- Tecan Spark™: Multimode Microplate Reader for quantification of nucleic acid, cell fluorescence, luminescence measurement, cell incubation, high-throughput screening up to 50×384 wells per measurement, cell imagining and counting - Luminex MagPix™: multiplex analysis system able to detect up to 50 analyzers per sample, it is applied to immunoassays, coupling protein assays and several serology techniques. - Freedom EVO75™ undertakes the repetitive fluid handling required in a high-performance experimental protocol with large number of wells or with multiple compounds. With eight-channel syringe pump and Capacitive liquid level detection with first tip it handles liquids in a volume range: 0.5 to 500 μl or 1 to 1000 μl. - FC500 Beckman Coulter cytometer offers advanced technology for cellular analysis. It offers the capacity to conduct 5-color analysis The FC500's CXP software offers automated flow cytometric analysis such as DNA analysis, immunophenotyping, and cellular subsetting. - Cell Culture units comprises of encompasses ten CO2 incubators for mammalian cell cultures, four incubators for bacterial cell cultures, two incubators for insect cell cultures and a Conviron incubator for silkworm maintenance (regulation of light and temperature), three for fungal cultures and two for plant growth, ten laminar-flow cabinets, a Ruskinn InVivo400 hypoxia workstation, one Coulter Z1 particle counter, and ten liquid nitrogen containers with capacity for over 10,000 samples.